Coding

Part:BBa_K3747113

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Periplasmic nitrate reductase B (napB) from Paracoccus denitrificans DSM 413

This gene is part of the nap operon from P. denitrificans DSM 413. Nap is the first enzyme of the denitrification pathway, converting nitrate (NO3-) into nitrite (NO2-). NapB is a small cytochrome c protein, which can transfer electrons to NapA [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 96
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] Coelho, C., & Romão, M. J. (2015). Structural and mechanistic insights on nitrate reductases. Protein Science, 24(12), 1901–1911. https://doi.org/10.1002/PRO.2801

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